Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor

Morita, Masatomo ; Ohnishi, Makoto ; Arakawa, Eiji ; Bhuiyan, N. A. ; Nusrin, Suraia ; Alam, Munirul ; Siddique, A. K. ; Qadri, Firdausi ; Izumiya, Hidemasa ; Balakrish Nair, G. ; Watanabe, Haruo (2008) Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor Microbiology and Immunology, 52 (6). pp. 314-317. ISSN 0385-5600

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1348-...

Related URL: http://dx.doi.org/10.1111/j.1348-0421.2008.00041.x

Abstract

A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.

Item Type:Article
Source:Copyright of this article belongs to Center for Academic Publications, Japan.
Keywords:Altered El Tor strain; Biotype; Cholera Toxin; Vibrio Cholerae O1
ID Code:80599
Deposited On:01 Feb 2012 12:54
Last Modified:01 Feb 2012 12:54

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