Genetic analysis of CTX prophages with special reference to ctxB and rstR alleles of Vibrio cholerae O139 strains isolated from Kolkata over a decade

Raychoudhuri, Amit ; Mukherjee, Piyali ; Ramamurthy, Thandavarayan ; Nandy, Ranjan K. ; Takeda, Yoshifumi ; Nair, Gopinath B. ; Mukhopadhyay, Asish K. (2010) Genetic analysis of CTX prophages with special reference to ctxB and rstR alleles of Vibrio cholerae O139 strains isolated from Kolkata over a decade FEMS Microbiology Letters, 303 (2). pp. 107-115. ISSN 0378-1097

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...

Related URL: http://dx.doi.org/10.1111/j.1574-6968.2009.01856.x

Abstract

Chronological analysis of 125 Vibrio cholerae O139 strains isolated during 1993-2005 in Kolkata revealed the prevalence of two new genotypes of cholera toxin (CT) and novel combinations of ctxB and rstR alleles resulting in variant CTX prophages. One of the new genotypes of ctxB, which first appeared in 1996 with the re-emerged V. cholerae O139 strains that had CTX Calcutta phage, was designated as genotype 4. In 1998, another new genotype, designated as genotype 5, was detected that prevailed mostly in CTX phages with El Tor rstR. The prototype El Tor CTX phage with genotype 3 gradually disappeared in O139, and since 2002 the predominant CTX prophages in O139 are Calcutta phages with genotype 4 and El Tor phages with genotype 5. Results showed that V. cholerae O139 strains of Kolkata, isolated over a decade, harboured CTX prophages in the large chromosome having no RS1 downstream of CTX prophage. During the course of its intermittent incidence over a decade, five types of O139 strains were detected based on CT genotypes. Such abrupt genetic changes in O139 strains might not favour its continued prevalence in human cases in Kolkata, India.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Vibrio Cholerae; CTX Prophages; rstR Alleles; ctxB; Cholera Toxin Genotype
ID Code:80569
Deposited On:01 Feb 2012 13:06
Last Modified:01 Feb 2012 13:06

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