Mukherjee, Koushik ; Sen, Joyeeta ; Chaudhuri, A. (2005) Common co-lipids, in synergy, impart high gene transfer properties to transfection-incompetent cationic lipids FEBS Letters, 579 (5). pp. 1291-1300. ISSN 0014-5793
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00145...
Related URL: http://dx.doi.org/10.1016/j.febslet.2004.11.116
Abstract
Efficacious cationic transfection lipids usually need either DOPE or cholesterol as co-lipid to deliver DNA inside the cell cytoplasm in non-viral gene delivery. If both of these co-lipids fail in imparting gene transfer properties, the cationic lipids are usually considered to be transfection inefficient. Herein, using both the reporter gene assay in CHO, COS-1 and HepG2 cells and the whole cell histochemical X-gal staining assay in representative CHO cells, we demonstrate that common co-lipids DOPE, Cholesterol and DOPC, when act in synergy, are capable of imparting improved gene transfer properties to a novel series of cationic lipids (1-5). Contrastingly, lipids 1-5 became essentially transfection-incompetent when used in combination with each of the pure co-lipid components alone.
Item Type: | Article |
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Source: | Copyright of this article belongs to Federation of European Biochemical Societies. |
Keywords: | Co-lipids; Non-viral Gene Therapy; Cationic Transfection Lipids; Lipofection; Lipid: DNA Interactions |
ID Code: | 8056 |
Deposited On: | 25 Oct 2010 10:12 |
Last Modified: | 16 May 2016 18:08 |
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