Entrainment properties of the locomotor activity rhythm of Drosophila melanogaster under different photoperiodic regimens

Abstract

In this paper we report the results of an experiment to assess how closely repeated brief light pulses (LPs) mimic the effects of 12:12?h light/dark (LD) cycles (PPc). The locomotor activity rhythm of individual fruit flies from a laboratory population of Drosophila melanogaster was monitored under four different photoperiodic regimens, created using 12?h of light and 12?h of darkness or brief light pulses (LPs). The phase relationship (?) and the stability (precision) of the locomotor activity rhythm during entrainment were estimated in order to compare the state of the circadian clocks under the four different photoperiodic regimens. The flies (n = 72) were subjected to four different LD cycles: (i) 12?h of light and 12?h of darkness (complete photoperiod, PPc); (ii) a single brief LP of 15?min duration presented close to the onset of activity (SLP-1); (iii) a single brief LP of 15?min duration administered close to the offset of activity (SLP-2); and (iv) two brief LPs administered 12?h apart (skeleton photoperiod, PPs). The locomotor activity rhythm of the flies was first monitored under constant darkness (DD) for about 10 days and then under the four different photoperiodic regimens for about 10 days, and finally in DD for the remainder of the experiment. The ? of the locomotor activity rhythm and its precision under PPc and PPs did not differ significantly, but they were significantly different from the SLP-1 and SLP-2 conditions. The results provide interesting insights into photoentrainment mechanisms of circadian clocks in D. melanogaster, and suggest that skeleton photoperiods, but not single brief LPs, mimic the actions of complete photoperiods.