Panchal, S. C. ; Madan, Anup ; Hosur, R. V. (1999) Cloning, expression and purification of the DNA binding domain of RFX protein Indian Journal of Biochemistry & Biophysics, 36 (6). pp. 429-432. ISSN 0301-1208
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Abstract
The RFX DNA binding domain (DBD) is a novel highly conserved motif belonging to a large number of dimer DNA binding proteins which have diverse regulatory functions in eukaryotic organisms. To characterize this novel motif, a 78mer polypeptide corresponding to the DBD of human hRFX (hrfX1/DBD), a prototypical member of the RFX family has been cloned and overproduced in Escherichia coli. A purification procedure using cation exchange chromatography has also been developed.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to National Institute of Science Communication and Information Resources. |
| ID Code: | 80102 |
| Deposited On: | 31 Jan 2012 04:21 |
| Last Modified: | 31 Jan 2012 04:21 |
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