Design, synthesis, and transfection biology of novel cationic glycolipids for use in liposomal gene delivery

Banerjee, Rajkumar ; Mahidhar, Yenugonda Venkata ; Chaudhuri, Arabinda (2001) Design, synthesis, and transfection biology of novel cationic glycolipids for use in liposomal gene delivery Journal of Medicinal Chemistry, 44 (24). pp. 4176-4185. ISSN 0022-2623

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Official URL: http://pubs.acs.org/doi/abs/10.1021/jm000466s

Related URL: http://dx.doi.org/10.1021/jm000466s

Abstract

The molecular structure of the cationic lipids used in gene transfection strongly influences their transfection efficiency. High transfection efficiencies of non-glycerol-based simple monocationic transfection lipids with hydroxyethyl headgroups recently reported by us (Banerjee et al. J. Med. Chem. 1999, 42, 4292-4299) are consistent with the earlier observations that the presence of hydroxyl functionalities in the headgroup region of a cationic lipid contributes favorably in liposomal gene delivery. Using simple sugar molecules as the source of multiple hydroxyl functionalities in the headgroup region of the transfection lipids, we have synthesized four novel simple monocationic transfection lipids, namely, 1-deoxy-1-[dihexadecyl(methyl)ammonio]-d-xylitol (1), 1-deoxy-1-[methyl(ditetradecyl)ammonio]-d-arabinitol (2), 1-deoxy-1-[dihexadecyl(methyl)ammonio]-d-arabinitol (3) and 1-deoxy-1-[methyl(dioctadecyl)ammonio]-d-arabinitol (4), containing hydrophobic aliphatic tails and the hydrophilic arabinosyl or xylose sugar groups linked directly to the positively charged nitrogen atom. Syntheses, chemical characterizations, and the transfection biology of these novel transfection lipids 1-4 are described in this paper. Lipid 1, the xylosyl derivative, showed maximum transfection on COS-1 cells. All the lipids showed transfection with cholesterol as colipid and not with dioleoylphosphatidylethanolamine (DOPE). Radioactive quantitation of free and complexed DNA combined with ethidium bromide exclusion measurements suggest that though nearly 70% of the DNA exists as complexed DNA, the DNA may not have condensed as was observed with other cationic lipids. Presence of additional (more than two) hydroxyl functionalities in the headgroup of the cationic lipids appears to have improved the transfection efficiency and made these lipids less cytotoxic compared to two-hydroxyl derivatives.

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Source:Copyright of this article belongs to American Chemical Society.
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Deposited On:25 Oct 2010 09:32
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