Trafficking motifs as the basis for two-compartment signaling systems to form multiple stable states

Abstract

Transport of molecules in cells is a central part of cell biology. Frequently such trafficking is not just for material transport, but also for information propagation, and serves to couple signaling circuits across cellular compartments. Here, I show that trafficking transforms simple local signaling pathways into self-organizing systems that span compartments and confer distinct states and identities to these compartments. I find that three motifs encapsulate the responses of most single-compartment signaling pathways in the context of trafficking. These motifs combine with different trafficking reactions to generate a diverse set of cellular functions. For example, trafficked bistable switches can oscillate or become quad- or tristable, depending on trafficking mechanisms and rates. Furthermore, the analysis shows how compartments participating in traffic can settle to distinct molecular compositions characteristic of distinct organelle identities. This general framework shows how the interplay between molecular movement and local reactions can generate many system functions, and give distinct identities to different parts of the cell.