Acetylene reduction assay for nitrogenase activity: gas chromatographic determination of ethylene per sample in less than one minute

David, K. A. V. ; Apte, S. K. ; Banerji, A. ; Thomas, Joseph (1980) Acetylene reduction assay for nitrogenase activity: gas chromatographic determination of ethylene per sample in less than one minute Applied and Environmental Microbiology, 39 (5). pp. 1078-1080. ISSN 0099-2240

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Official URL: http://aem.asm.org/cgi/content/abstract/39/5/1078

Abstract

Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatographically at the rate of about 13 samples in 10 min.

Item Type:	Article
Source:	Copyright of this article belongs to American Society for Microbiology..
ID Code:	784
Deposited On:	25 Sep 2010 04:43
Last Modified:	16 May 2016 11:59

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