Gene expression during somatic embryogenesis - recent advances

Abstract

Somatic embryogenesis is a remarkable illustration of the dictum of plant totipotency. During embryogenic induction of cells, there is differential gene expression resulting in synthesis of new mRNAs and proteins. This genetic information in turn elicits diverse cellular and physiological responses that are involved in 'switching over' of the developmental programme of the somatic cells. Various model systems have been widely investigated to understand the mechanisms of gene regulation during this developmental process, and an array of genes activated or differentially expressed during somatic embryogenesis have been isolated employing various molecular techniques. Nonetheless, the precise mechanisms controlling plant gene expression and the detailed steps by which these genes direct the plantspecific process of somatic embryogenesis remain far from being clearly understood. Thus, future trends involve characterization of development-specific genes during somatic embryogenesis to provide a deeper insight in understanding the mechanisms involved during differentiation of somatic cells and phenotypic expression of cellular totipotency in higher plants.