Sequence studies of Indian Peanut Clump furovirus RNA

Abstract

Indian peanut clump virus (IPCV) has a bi-partite genome and is transmitted by Polymyxa graminis: it has been classified in the furovirus genus. Cloning and sequencing of cDNA made to RNA from IPCV particles has revealed that the 5'-proximal ORF on RNA-2 codes for the coat protein and that RNA-1 codes for a putative methyl transferase gene. The 3'-halves of each RNA have yet to be sequenced. IPCV coat protein is 61% identical to the coat protein of peanut clump virus from West Africa (PCV). This value, together with the lack of serological relatedness between them, confirms that IPCV and PCV should be considered as separate viruses. Comparisons among the coat proteins of a range of rod-shaped viruses showed that IPCV and PCV coat proteins resembled that of barley stripe mosaic hordeivirus more than those of other furoviruses., Indian peanut clump virus (IPCV) has a bi-partite genome and is transmitted by Polymyxa graminis: it has been classified in the furovirus genus. Cloning and sequencing of cDNA made to RNA from IPCV particles has revealed that the 5'-proximal ORF on RNA-2 codes for the coat protein and that RNA-1 codes for a putative methyl transferase gene. The 3'-halves of each RNA have yet to be sequenced. IPCV coat protein is 61% identical to the coat protein of peanut clump virus from West Africa (PCV). This value, together with the lack of serological relatedness between them, confirms that IPCV and PCV should be considered as separate viruses. Comparisons among the coat proteins of a range of rod-shaped viruses showed that IPCV and PCV coat proteins resembled that of barley stripe mosaic hordeivirus more than those of other furoviruses.