Das, Tapas ; Mandal, N. C. (1986) Structure and function of the repressor of bacteriophage lambda III. Molecular cloning of the high-affinity mutant cl gene of lambda and studies of the properties of the clones Molecular and General Genetics MGG, 204 (3). pp. 540-542. ISSN 0026-8925
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Official URL: http://www.springerlink.com/content/u880861812h232...
Related URL: http://dx.doi.org/10.1007/BF00331037
Abstract
The high-affinity mutant cI gene of λ cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively, the level of repressor in a monolysogen of λ cIha. Results of the study of certain properties of the bacteria carrying these plasmids suggest that the ha repressor also has a higher affinity for the virulent mutant operators as well as the prm promoter of λ.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Bacteriophage Lambda; High-affinity Mutant Ci Gene; Cloning - Ha Repressor Of Lambda |
| ID Code: | 74586 |
| Deposited On: | 17 Dec 2011 10:03 |
| Last Modified: | 17 Dec 2011 10:03 |
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