X-ray studies on crystalline complexes involving amino acids. IV. The structure of L-arginine L-ascorbate

Abstract

L-Arginine ascorbate, C₆H_I5N₄O₂⁺· C₆H₇O₆^-, a 1:1 crystalline complex between the amino acid arginine and the vitamin ascorbic acid, crystallizes in the monoclinic space group P2₁ with two formula units in a cell of dimensions a = 5.060 (8), b = 9.977 (9), c = 15.330 (13) Å, β = 97.5 (2) °. The structure was solved by the symbolic addition procedure and refined to an R of 0.067 for 1501 photographically observed reflections. The conformation of the arginine molecule in the structure is different from any observed so far. The present structure provides the first description of the ascorbate anion unaffected by the geometrical constraints and disturbances imposed by the requirements of metal coordination. The lactone group and the deprotonated enediol group in the anion are planar and the side chain assumes a conformation which appears to be sterically the most favourable. In the crystals, the arginine molecules and the ascorbate anions aggregate separately into alternating layers. The molecules in the arginine layer are held together by interactions involving α-amino and ~t-carboxylate groups, a situation analogous to that found in proteins. The two layers of unlike molecules are interconnected primarily through the interactions of the side-chain guanidyl group of arginine with the ascorbate ion. These involve a specific ion-pair interaction accompanied by two convergent hydrogen bonds and another pair of nearly parallel hydrogen bonds.