Quality & quantity of dietary protein & acute endosulphan metabolic toxicity in rat liver microsomes

Abstract

The effect of feeding rice diets with and without lysine and threonine supplementation and endosulphan administration was studied on rat liver microsomal lipids, and two mixed function oxidases (viz., aniline hydroxylase and aminopyrine N-demethylase). These groups were compared with rats fed 20 per cent casein diet and given endosul phan. Endosulphan administration increased liver microsomal protein in animals fed ad libitum. Activities of both enzymes were more in rats fed ad libitum, as compared to pair-fed rats. Endosulphan administration increased the activity of aniline hydroxylase more In the deficient group, as compared to the supplemented group. The activity of aminopyrine N-demethylase was increased significantly in the supplemented and deficient groups following endosulphan administration. Total phospholipids, phosphatidylcholine and phosphatidylethanolamine contents were higher in pair-fed rats as compared to rats fed ad libitum. the cholesterol content in the supplemented and casein groups of ad libitum fed rats were nearly comparable. However, in the endosulphan treated deficient group cholesterol level was significantly higher than the untreated deficient group.