Singh, Anubha ; Singh, Yogendra ; Pine, Richard ; Shi, Lanbo ; Chandra, Ramesh ; Drlica, Karl (2006) Protein kinase I of Mycobacterium tuberculosis: cellular localization and expression during infection of macrophage-like cells Tuberculosis, 86 (1). pp. 28-33. ISSN 1472-9792
Full text not available from this repository.
Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.tube.2005.04.002
Abstract
Protein kinase I of Mycobacterium tuberculosis, which has an unusual amino acid composition in its catalytic loop, displayed autophosphorylation and transphosphorylation activity. Immunoblot analysis of sub-cellular fractions of M. tuberculosis, using anti-PknI antibodies raised in rabbits, showed that PknI localizes to the bacterial cytosol. In contrast, PknA was membrane-bound. Relative expression of pknI, when measured by combining molecular beacons and RT-PCR, decreased during infection of THP-1 human macrophages. Expression of pknA and pknB was upregulated during infection. Thus PknI represents a group of protein kinases that is distinct from the more extensively studied enzymes PknA and PknB.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Protein Kinase A; Protein Kinase I; Mycobacterium tuberculosis; Molecular Beacons; Transcription |
| ID Code: | 74243 |
| Deposited On: | 09 Dec 2011 05:34 |
| Last Modified: | 09 Dec 2011 05:34 |
Repository Staff Only: item control page
