Koundal, K. R. ; Mehta, S. L. ; Sharma, R. P. (1989) Construction of Chickpea Genomic Library Indian Journal of Experimental Biology, 27 (10). pp. 858-860. ISSN 0019-5189
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Abstract
For construction of chickpea genomic library, DNA was isolated, purified on CsCl gradient and size fractionated into 15-20 Kb fragments after restriction with Sau 3A. These fragments were ligated to phage lambda (EMBL-3) vector and the recombinant molecules packaged in vitro into viable phage particles. The recombinant phages were obtained as phages on a P2 lysogen of E. coli (Spi- selection) and amplified to establish a permanent library. This is the first report of the construction of chickpea genomic library.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to National Institute of Science Communication and Information Resources. |
| ID Code: | 74067 |
| Deposited On: | 08 Dec 2011 12:16 |
| Last Modified: | 08 Dec 2011 12:16 |
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