Cloning of rice DNA and identification of transfer-RNA gene clones

Vasavada, H. A. ; Thomas, George ; Padayatty, J. D. (1981) Cloning of rice DNA and identification of transfer-RNA gene clones Current Science, 50 (20). pp. 887-888. ISSN 0011-3891

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Official URL: http://www.ias.ac.in/j_archive/currsci/50/20/887-8...

Abstract

DNA from 48 hr germinated rice embroys was cut with restriction endonuclease Bam H1 and cloned to the Bam site on plasmid pBR 322. The clone containing recombinant DNA were selected by their sensitivity to tetracycline and resistance to ampicillin. Using 32P-;abelled rice embryo tRNA as a probe two clone were identified to contain tRNA genes by colony hybridization.

Item Type:Article
Source:Copyright of this article belongs to Current Science Association.
ID Code:73685
Deposited On:07 Dec 2011 05:42
Last Modified:18 May 2016 18:24

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