Chattopadhyay, Amitabha ; Rukmini, R. (1993) Restricted mobility of the sole tryptophan in membrane-bound melittin FEBS Letters, 335 (3). pp. 341-344. ISSN 0014-5793
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...
Related URL: http://dx.doi.org/10.1016/0014-5793(93)80415-Q
Abstract
In spite of numerous studies, there appears to be no consensus regarding the orientation and aggregation state of membrane-bound melittin. We report here the restricted environment of the sole tryptophan residue in membrane-bound melittin using environment-induced effects on the rates of solvent relaxation. When incorporated into unilamellar vesicles of dioleoyl-sn-glycero-3-phosphocholine (DOPC), melittin exhibits a red edge excitation shift (REES) of 5 nm. In addition, fluorescence polarization of melittin m the membrane shows both excitation and emission wavelength dependence. Taken together, these observations indicate that the tryptophan residue of melittin is located in a motionally restricted region in the membrane.
Item Type: | Article |
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Source: | Copyright of this article belongs to Federation of European Biochemical Societies. |
Keywords: | Restricted Tryptophan Environment; Membrane-bound Melittin; Red Edge Excitation Shift; Fluorescence Polarization |
ID Code: | 7300 |
Deposited On: | 25 Oct 2010 11:53 |
Last Modified: | 16 May 2016 17:31 |
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