Gupta, S. K. ; Bagavant, H. ; Koothan, P. T. ; Talwar, G. P. ; Yurewicz, E. C. ; Sacco, A. G. (1992) Characteristics of monoclonal antibodies against porcine zona pellucida-3 and their functional relevance Indian Journal of Experimental Biology, 30 (11). pp. 1000-1005. ISSN 0019-5189
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Abstract
Seven monoclonal antibodies (MAs) against 55 kDa glycoprotein family of porcine zona pellucida (ZP3) reacting with either ZP3 α (MA-7, MA-27, MA-28) or ZP3 β (MA-1, MA-2, MA-11, MA-30) have been described. MA-1, -2, -27, -28 and -30 do not recognize carbohydrate determinants as shown by their reactivity to the deglycosylated (DG) ZP3 alpha and ZP3 beta. Indirect immunoperoxidase studies showed that all MAs reacted with zona pellucida from porcine and monkey ovaries. Only MA-1 and -27 reacted with ZP from rabbit ovary as well, while none of the MAs recognised mouse ZP, MA-7, -11, -27, -28 and -30 inhibited in vitro, the zona lysis by trypsin as well as the binding of ZP3 to sperm membrane vesicle as investigated by ELISA.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to National Institute of Science Communication and Information Resources. |
| ID Code: | 72178 |
| Deposited On: | 28 Nov 2011 06:07 |
| Last Modified: | 28 Nov 2011 06:07 |
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