Kurpisz, Maciej ; Gupta, Satish K. ; Fulgham, David L. ; Alexander, Nancy J. (1988) Production of large amounts of mouse polyclonal antisera Journal of Immunological Methods, 115 (2). pp. 195-198. ISSN 0022-1759
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0022-1759(88)90288-8
Abstract
A simple and inexpensive method for producing larger volumes of mouse polyclonal antisera is described. Groups of BALB/c mice were immunized with different human sperm antigens classified according to molecular weight. Mice were immunized twice with the antigen, twice pristane pretreated, and then injected with a non-antibody-secreting myeloma cell line to induce ascites formation. Antibody activity of the ascites fluid approximated that of the serum. Thus, one mouse can provide about six times the amount of antibody-containing fluid usually obtained after conventional immunization. This method also may be applicable for ascites fluid production in nude mice.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Ascites; Polyclonal Antiserum; Sperm Antigen |
| ID Code: | 72169 |
| Deposited On: | 28 Nov 2011 06:06 |
| Last Modified: | 28 Nov 2011 06:06 |
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