An ATP-independent catenating enzyme from the kinetoplast hemoflagellate Leishmaniadonovani

Chakrabortya, A. K. ; Majumder, H. K. (1991) An ATP-independent catenating enzyme from the kinetoplast hemoflagellate Leishmaniadonovani Biochemical and Biophysical Research Communications, 180 (1). pp. 279-285. ISSN 0006-291X

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/S0006-291X(05)81289-6

Abstract

An enzyme from Leishmaniadonovani that catenates monomeric pBR322 into huge catenanes has been isolated and characterized. The enzyme also decatenates kinetoplast DNA networks into covalently closed monomeric circles and relaxes supercoiled pBR322. The catenation, decatenation and relaxation reactions do not require ATP. The formation of topological isomers of unique linking numbers suggest that the enzyme is a type II DNA topoisomerase.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:72078
Deposited On:03 Dec 2011 06:53
Last Modified:03 Dec 2011 06:53

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