Santhosh, Ramachandran Sarojini ; Pandian, Shunmugiah Karutha ; Lini, Nirmala ; Shabaana, Abdul Khader ; Nagavardhini, Avuthu ; Dharmalingam, Kuppamuthu (2005) Cloning of mce1 locus of Mycobacterium leprae in Mycobacterium smegmatis mc2 155 SMR5 and evaluation of expression of mce1 genes in M. smegmatis and M. leprae FEMS Immunology and Medical Microbiology, 45 (2). pp. 291-302. ISSN 0928-8244
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Official URL: http://onlinelibrary.wiley.com/doi/10.1016/j.femsi...
Related URL: http://dx.doi.org/10.1016/j.femsim.2005.05.004
Abstract
Plasmid pSET152 is a broad host range mobilizable vector which integrates into streptomyces chromosome utilizing att site and int function of ØC31. Transformation of this plasmid into Mycobacterium smegmatis mc2 155 SMR5 gave stable transformants carrying the pSET152 as an integrated copy. Integration occurred at the cross over sequence 5'TTG disrupting the gatA gene (Glu-tRNAGln amidotransferase subunitA), which is non-essential under conditions used. Recombinant pSET152 plasmids carrying mce1 locus of Mycobacterium leprae were used to construct M. smegmatis transformants carrying the mce1 locus in their chromosome. RT-PCR analysis revealed specific transcripts of M. leprae mce in M. smegmatis. The transcribed mRNA carried intergenic regions between genes of mce1 locus indicating that mce1 locus is an operon. Examination of M. leprae specific mRNA from lepromatous leprosy patient's biopsy showed that mce locus is transcribed as an operon in the pathogen also.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| ID Code: | 70406 |
| Deposited On: | 17 Nov 2011 03:47 |
| Last Modified: | 17 Nov 2011 03:47 |
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