Chattopadhyay, Amitabha (1990) Depth profiling in membranes by fluorescence quenching Journal of Biosciences, 15 (3). pp. 143-144. ISSN 0250-5991
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Official URL: http://www.ias.ac.in/jarch/jbiosci/15/143-144.pdf
Related URL: http://dx.doi.org/10.1007/BF02703874
Abstract
Membrane penetration depth is an important parameter in relation to membrane structure and organization. A methodology has been developed to analyze the membrane penetration depths of fluorescent molecules or groups utilizing differential fluorescence quenching caused by membrane embedded spin-label probes located at different depths. The method involves determination of the parallax in the apparent location of fluorophores, detected when quenching by phospholipids spin-labelled at two different depths is compared. By use of relatively simple algebraic expressions, the method allows calculation of depth in λ . This method has been used to determine the location of fluorophores in NBD-labelled lipids and anthroyloxy-labelled fatty acids in model membranes and of the membrane embedded tryptophan residues in the reconstituted nicotinic acetylcholine receptor.
Item Type: | Article |
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Source: | Copyright of this article belongs to Indian Academy of Sciences. |
Keywords: | Fluorescence Quenching; Spin Labelled Phospholipids; Membrane Penetration Depth |
ID Code: | 6985 |
Deposited On: | 26 Oct 2010 04:49 |
Last Modified: | 16 May 2016 17:14 |
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