Chain-terminating natural mutations affect the function of activating KIR receptors 3DS1 and 2DS3

Luo, Lihui ; Du, Zeying ; Sharma, Surendra K. ; Cullen, Rebecca ; Spellman, Stephen ; Reed, Elaine F. ; Rajalingam, Raja (2007) Chain-terminating natural mutations affect the function of activating KIR receptors 3DS1 and 2DS3 Immunogenetics, 59 (10). pp. 779-792. ISSN 0093-7711

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Official URL: http://www.springerlink.com/content/qt47r785k84221...

Related URL: http://dx.doi.org/10.1007/s00251-007-0239-1

Abstract

To determine the nucleotide polymorphism of activating killer-cell immunoglobulin-like receptors (aKIR) 3DS1 and 2DS3, we developed a novel direct-sequencing method and analyzed DNA samples of 175 KIR3DS1+ individuals and 72 KIR2DS3+ individuals from the white population. The putative ligand-binding extracellular immunoglobulin (Ig)-like domains of these aKIR receptors are highly conserved, a scenario contrary to inhibitory KIRs that recognize polymorphic human leukocyte antigen (HLA) class I molecules. Null alleles 3DS1049N and 2DS3003N that do not express cell-surface receptors were discovered, and they occur commonly in whites (3DS1049N = 2%; 2DS3003N = 0.8%). Sequence-specific polymerase chain reaction (PCR) detecting these null alleles is negative with DNA from nonwhite subjects, suggesting that these null alleles are specific to whites and probably originated after the colonization of modern humans in Europe.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:KIR Gene; HLA; Natural Killer Cells; Null Alleles; Gene Regulation
ID Code:69365
Deposited On:10 Nov 2011 10:14
Last Modified:10 Nov 2011 10:14

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