Kalsi, Gurpreet ; Das, Hasi R. ; Babu, Cherukuri R. ; Das, Rakha H. (1992) Isolation and characterization of a lectin from peanut roots Biochimica et Biophysica Acta (BBA) - General Subjects, 1117 (2). pp. 114-119. ISSN 0304-4165
Full text not available from this repository.
Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0304-4165(92)90067-5
Abstract
A glucose-specific lectin has been purified to apparent homogeneity from 7-day-old peanut (Arachis hypogaea) roots by affinity chromatography on a Sephades G-50. The lectin has a 66 kDa native molecular mass and a 33 kDa subunit molecular mass as revealed by native and denaturing sodium dedecyl sulphate-polycrylamide gel electrophoresis, respectively. The purified lectin, gives a single precipitin line with the antiserum produced against 7-day-old root extract and shows 5 bands in the pH range of 4.4–5.4 in the isoelectric focusing gel. The glucose-specific lectin activity in the peanut roots appears from the fourth day onwards. Lipopolysaccharides isolated from the host specific Rhizobium strain are a 68-fold more potent inhibitor of the lectin as compared to glucose.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Root lectin; Affinity Chromatography; (Peanut); (Arachis hypogaea) |
| ID Code: | 69188 |
| Deposited On: | 22 Nov 2011 10:55 |
| Last Modified: | 22 Nov 2011 10:55 |
Repository Staff Only: item control page
