M150 modulates the costimulatory signals delivered by B cells to T cells and enhances their ability to help B cells

Abstract

There is a prerequirement of at least two sets of signals delivered by the antigen-presenting cell (APC) for the optimal activation of T helper (Th) cells. The First signal is provided by the engagement of T cell receptor with the antigen-MHC class II complex, followed by a second stimulus in the form of costimulatory signals. In the present study, we provide evidence that in a T-dependent antigen-driven system, the signals generated by hapten-specific B cells to stimulate Th cells for the secretion of interleukin-2 (IL-2), interferon-γ (IFN-γ ), and IL-4 were differentially modified by M150, a 150-kDa molecule expressed on the surface of macrophages. When ovalbumin-specific Th cells were cultured in the presence of 2,4,6 trinitrophenol (TNP)-specific B cells, M150 significantly increased the proliferation of Th cells and the secretion of IL-2 and IFN- γ and decreased the production of IL-4. Further, Th cells stimulated with M150 acquired improved ability to help B cells, resulting in an increase in the number of antibody-secreting cells and in the production of TNP-specific IgG2a antibodies. M150 possibly promotes Th1-like cell activity, as evidenced by predominant secretion of IL-2, IFN-γ , and IgG2a but not IL-4 and IgG1.