Antibodies to guanylic acid: fractionation and specificities

Abstract

Guanylic-acid-specific antibodies were elicited in rabbits) using as immunogen pG linked through 5'-phosphate to thyroglobulin. Specificity and affinity of antibodies to nucleotides) nucleosides, DNA, and RNA were studied by their binding to radioactive ligands and competition experiments . Guanylic-acid-specific antibodies do not bind to deoxyguanylic acid and have an average association constant of 10⁷ M^-1 at 4°C Binding of the antibodies to ³H-RNA is G-specifiC. The antibodies do not bind to ³²P-ssDNA or ³²P-dsDNA. The pG-specific antibodies could be separated into different fractions by affinity chromatography. These fractions) though specific to pG) differ in their cross-reactivities to nucleosides and nucleotides.