New model systems to study DNA-protein recognition mechanisms

Jacob, Alexander ; Jacob, T. M. (1985) New model systems to study DNA-protein recognition mechanisms FEBS Letters, 189 (1). pp. 81-84. ISSN 0014-5793

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...

Related URL: http://dx.doi.org/10.1016/0014-5793(85)80846-2

Abstract

dpG antibodies were fractionated on a cellulose-double-stranded DNA column. The flow-through fraction bound denatured DNA but not double-stranded DNA (dsDNA). The dpG-eluted fraction bound dsDNA preferentially. The results show that proteins can recognise dpG in DNA by different mechanisms, some involving DNA unwinding.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:dpC Antibody; Fractionation; dsDNA Binding; dsDNA-Cellulose Chromatography; dpG Conformation
ID Code:67969
Deposited On:02 Nov 2011 03:01
Last Modified:02 Nov 2011 03:01

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