A highly specific heterologous enzyme-linked immunosorbent assay for measuring testosterone in plasma using antibody-coated immunoassay plates or polypropylene tubes

Rassaie, Mohammad J. ; Kumari, Lakshmi G. ; Pandey, Pramod K. ; Gupta, Nandita ; Kochupillai, N. ; Grover, P. K. (1992) A highly specific heterologous enzyme-linked immunosorbent assay for measuring testosterone in plasma using antibody-coated immunoassay plates or polypropylene tubes Steroids, 57 (6). pp. 288-294. ISSN 0039-128X

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0039-128X(92)90062-E

Abstract

A highly specific and sensitive enzyme-linked immunosorbent assay, using a heterologous combination of antiserum raised against testosterone-3-(O-carboxymethyl) oxime-bovine serum albumin and penicillinase-labeled testosterone-11β -carboxy methyl ether, was developed for measuring testosterone in human plasma. Immunoassay plates (96 wells) provided a sensitivity of 2.5 pg/well. This was achieved by maintaining the molar ratios of steroid to enzyme between 10 and 20. The assay was very specific for testosterone and did not show any cross-reaction with the related C19 steroids tested. Replacement of immunoassay plates with the locally available polypropylene tubes raised the detection limits to 25 pg/tube, but improved the range of doses of testosterone that could be measured up to 10,000 pg. The antiserum to testosterone derivative was linked to both immunoassay plates and polypropylene tubes through immunochemical bridges. Comparison of testosterone values of 52 plasma specimens obtained by both solid phase methods with those of radioimmunoassay showed good correlation.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Testosterone in Plasma; Radioimmunoassay; Enzyme-linked Immunosorbent Assay (ELISA); Steroid; Plasma Testosterone; Heterologous ELISA; Penicillinase
ID Code:67520
Deposited On:31 Oct 2011 06:13
Last Modified:31 Oct 2011 06:13

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