Pathotypes of diarrheagenic Escherichia coli in children attending a tertiary care hospital in South India

Rajendran, Priya ; Ajjampur, Sitara Swarna Rao ; Chidambaram, Divya ; Chandrabose, Gunasekaran ; Thangaraj, Bhuvaneswari ; Sarkar, Rajiv ; Samuel, Prasanna ; Rajan, Deva Prasanna ; Kang, Gagandeep (2010) Pathotypes of diarrheagenic Escherichia coli in children attending a tertiary care hospital in South India Diagnostic Microbiology and Infectious Disease, 68 (2). pp. 117-122. ISSN 0732-8893

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.diagmicrobio.2010.06.003

Abstract

The prevalence of diarrheagenic Escherichia coli (DEC) in children under 5 years was studied in children with diarrhea and controls in South India. Four polymerase chain reaction (PCR) "schemes" were used to detect genes of the 6 pathotypes of DEC. In 394 children with diarrhea, 203 (52%) DEC infections were found. Among the 198 controls, 126 (63%) DEC infections were found. Enteroaggregative E. coli was the most common pathotype by multiplex PCR both in cases (58, 14.7%) and controls (47, 23.7%), followed by enteropathogenic E. coli seen in 10% cases and 8% of controls. Enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), and diffusely adherent E. coli (DAEC) were found in 4.1%, 2.0%, 1.0%, and 0.5% of cases, respectively. ETEC was found in 2.5% of controls, but EHEC, EIEC, and DAEC were not detected. Overall, no single assay worked well, but by discounting genes with a pathogenicity index of less than 1, it was possible to use the PCR assays to identify DEC in 75/394 (19%) cases and 12/198 (6.1%) controls, while mixed infection could be identified in 8/394 (2%) cases and 2/198 (1%) controls.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Diarrheagenic E. Coli; Multiplex PCR; Virulence Genes; Adherence Assays
ID Code:67015
Deposited On:28 Oct 2011 11:22
Last Modified:28 Oct 2011 11:22

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