Marrugo, Javier ; Marsh, David G. ; Ghosh, Balaram (1996) The conserved lymphokine element-0 in the IL5 promoter binds to a high mobility group-1 protein Molecular Immunology, 33 (14). pp. 1119-1125. ISSN 0161-5890
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/S0161-5890(96)00073-9
Abstract
The conserved lymphokine elements-0 (CLE0) in the IL5 promoter is essential for the expression of IL-5. Here, we report the cloning and expression of a cDNA encoding a novel CLE0-binding protein, CLEBP-1 from a mouse Th2 clone, D10.G4.1. Interestingly, it was found that the CLEBP1 cDNA sequence was almost identical to the sequences of known high mobility group-1 (HMG1) cDNAs. When expressed as a recombinant fusion protein in Escherichia coli, CLEBP-1 was shown to bind to the IL5-CLE0 element in electrophoretic mobility-shift assays (EMSA) and southwestern blot analysis. The CLEBP-1 fusion protein cross-reacts with and-HMG-½ in Western blot analysis. It also binds to the CLE0 elements of IL4, GMCSF and GCSF genes. CLEBP-1 and closely related HMG-1 and HMG-2 proteins may play key roles in facilitating the expression of the lymphokine genes that contain CLE0 elements.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Interleukin-5; Conserved Lymphokine Element-0; High Mobility Group-1 Protein; Promoter; D10; G4.1 |
| ID Code: | 66081 |
| Deposited On: | 21 Oct 2011 03:28 |
| Last Modified: | 21 Oct 2011 03:28 |
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