Gupta, Pankaj ; Balwani, Sakshi ; Kumar, Sarvesh ; Aggarwal, Neha ; Rossi, Miriam ; Paumier, Sarah ; Caruso, Francesco ; Bovicelli, Paolo ; Saso, Luciano ; DePass, Anthony L. ; Prasad, Ashok K. ; Parmar, Virinder S. ; Ghosh, Balaram (2010) β-sitosterol among other secondary metabolites of Piper galeatum shows inhibition of TNFα-induced cell adhesion molecule expression on human endothelial cells Biochimie, 92 (9). pp. 1213-1221. ISSN 0300-9084
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.biochi.2010.06.005
Abstract
A phytochemical investigation of the stems of Piper galeatum yielded one novel amide, 1-(3'-hydroxy-5'-methoxycinnamoyl)-piperidine (5) along with four known compounds, i.e. β-sitosterol (1), cyclostachine-A (2), piperine (3) and piperolein-B (4). The structures of all the five compounds, isolated for the first time from this plant were unambiguously established on the basis of their detailed spectral analysis. The structure of cyclostachine-A (2) was confirmed by X-ray crystallographic studies and structures of known compounds were confirmed by comparison of their physical and/or chemical data with those reported in the literature, which were in complete agreement. Additionally, the crude extracts as well as the isolated pure compounds were screened for their activity to inhibit TNFα (tumour necrosis factor-α)- induced expression of cell adhesion molecule ICAM-1 (intercellular adhesion molecule-1) on the surface of human umbilical vein endothelial cells (HUVECs). Among all, β-sitosterol (1) was found to be the most active compound, which was taken for further studies. β-sitosterol also significantly inhibited the TNFα-induced expression of VCAM-1 and E-selectin, which also play key role in various inflammatory diseases. The functional correlation of cell adhesion molecules inhibition was assessed by cell adhesion assay using human neutrophils. We found that β-sitosterol significantly blocks the adhesion of neutrophils to endothelial monolayer. To elucidate the molecular mechanism of inhibition of cell adhesion molecules, we investigated the status of nuclear transcription factor-κB (NF-κB) and were able to establish that β-sitosterol significantly blocked the TNFα-induced activation of NF-κB.
Item Type: | Article |
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Source: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Piper galeatum; 1-(3'-hydroxy-5'-methoxycinnamoyl)-piperidine; Anti-inflammatory Activity; ICAM-1; VCAM-1; E-selectin |
ID Code: | 66078 |
Deposited On: | 21 Oct 2011 03:44 |
Last Modified: | 21 Oct 2011 03:44 |
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