Dendritic poly(ether imine) based gene delivery vector

Padinjaremattathil Thankappan, Ullas ; Narayan Madhusudana, Shampur ; Desai, Anita ; Jayamurugan, Govindasamy ; Rajesh, Yamajala B. R. D. ; Jayaraman, Narayanaswamy (2011) Dendritic poly(ether imine) based gene delivery vector Bioconjugate Chemistry, 22 (2). pp. 115-119. ISSN 1043-1802

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Official URL: http://pubs.acs.org/doi/abs/10.1021/bc1003108

Related URL: http://dx.doi.org/10.1021/bc1003108

Abstract

The nonviral vector based gene delivery approach is attractive due to advantages associated with molecular-level modifications suitable for optimization of vector properties. In a new class of nonviral gene delivery systems, we herein report the potential of poly(ether imine) (PETIM) dendrimers to mediate an effective gene delivery function. PETIM dendrimer, constituted with tertiary amine branch points, n-propyl ether linkers and primary amines at their peripheries, exhibits significantly reduced toxicities, over a broad concentration range. The dendrimer complexes pDNA effectively, protects DNA from endosomal damages, and delivers to the cell nucleus. Gene transfection studies, utilizing a reporter plasmid pEGFP-C1 and upon complexation with dendrimer, showed a robust expression of the encoded protein. The study shows that PETIM dendrimers are hitherto unknown novel gene delivery vectors, combining features of poly(ethylene imine)-based polymers and dendrimers, yet are relatively nontoxic and structurally precise.

Item Type:Article
Source:Copyright of this article belongs to American Chemical Society.
ID Code:65989
Deposited On:20 Oct 2011 07:13
Last Modified:20 Oct 2011 07:13

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