Reconstitution of denatured E. coli alkaline phosphatase with E. coli ribosome

Das, B. ; Gupta, C. D. (1992) Reconstitution of denatured E. coli alkaline phosphatase with E. coli ribosome Indian Journal of Biochemistry & Biophysics, 29 (6). pp. 512-515. ISSN 0301-1208

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Abstract

E. coli alkaline phosphatase was denatured by physical/chemical means. In vitro reconstitution of this denatured enzyme was assisted by 70S E. coli ribosome, as shown by the recovery of its catalytic competence. Almost total recovery of activity of the totally inactivated enzyme was obtained in presence of equimolar concentration of 70S ribosome at 50 ° C.

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Deposited On:19 Oct 2011 09:09
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