Transgenic expression of hevein, the rubber tree lectin, in Indian mustard confers protection against Alternaria brassicae

Abstract

The antifungal effect of hevein, the chitin binding lectin from rubber plant (Hevea brasiliensis), has been analyzed in transgenic plants for potential control of Alternaria blight in Indian mustard caused by Alternaria brassicae. A cDNA encoding hevein was transferred into Indian mustard (Brassica juncea cv. RLM-198). Southern analysis of the putative transgenics showed integration of the transgene. Northern and Western analyses proved that the integrated transgene is expressed in the transgenics. In whole plant bioassay under glasshouse conditions, transgenics were found to possess parameters that are associated with resistance such as longer incubation and latent period; smaller necrotic lesion size; lower disease intensity and delayed senescence.