Dinesh Kumar, V. ; Kirti, P. B. ; Sachan, J. K. S. ; Chopra, V. L. (1994) Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.) Plant Cell Reports, 13 (8). pp. 468-472. ISSN 0721-7714
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Official URL: http://www.springerlink.com/content/83a14c44395aa1...
Related URL: http://dx.doi.org/10.1007/BF00231969
Abstract
Efficient plant regeneration via somatic embryogenesis has been developed in chickpea cultivar C235. Leaf explants, on MS medium supplemented with 1.25 mg/l 2,4-D and 0.25 mg/l kinetin, yielded somatic embryos with high efficiency during dark incubation. MS medium supplemented with B5 vitamins, 0.125 mg/l IBA and 2 mg/l BAP was found suitable for embryo maturation. The well formed embryos germinated into plantlets on basal B5 medium supplemented with 0.25 mg/l BAP. Further development into healthy plantlets was obtained on basal B5 medium. Hardened plantlets produced normal, fertile plants upon transfer to soil.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer-Verlag. |
| ID Code: | 6492 |
| Deposited On: | 20 Oct 2010 10:39 |
| Last Modified: | 28 May 2011 06:43 |
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