Sinha, Birandra K. ; Singh, Yogendra ; Krishna, Gopal (1986) Formation of superoxide and hydroxyl radicals from 1-methyl-4-phenylpyridinium ion(MPP+): reductive activation by NADPH cytochrome P-450 reductase Biochemical and Biophysical Research Communications, 135 (2). pp. 583-588. ISSN 0006-291X
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0006-291X(86)90033-1
Abstract
Formation of free radical intermediates from 1--methyl-4-phenylpyridinium ion(MPP+) has been studied using spin-trapping techniques. Incubation of MPP+ with purified NADPH cytochrome P-450 reductase and NADPH under anaerobic conditions failed to produce any detectable radical intermediates. However, in the presence of air and a spin-trap, a significant stimulation of superoxide and hydroxyl radicals was detected. Formation of these toxic radicals from MPP+ was inhibited by superoxide dismutase, catalase, and ethanol. Under identical conditions, however, considerably less of these radicals were formed with MPP+ in comparison to paraquat, a lung toxin containing two pyridinium moieties.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 64845 |
| Deposited On: | 15 Oct 2011 12:45 |
| Last Modified: | 15 Oct 2011 12:45 |
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