Panchal, Manoj ; Muralidhar, K. (2007) Purification of monomeric prolactin charge isoform from buffalo pituitaries Preparative Biochemistry and Biotechnology, 38 (1). pp. 94-104. ISSN 1082-6068
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Official URL: http://www.tandfonline.com/doi/abs/10.1080/1082606...
Related URL: http://dx.doi.org/10.1080/10826060701774395
Abstract
A standard protocol for isolation of buffalo prolactin (buPRL) was modified at the alcohol precipitation step. This modification could separate lower molecular weight prolactin from the higher molecular weight prolactin (PRL). Reloading the prolactin onto a Sephacryl S-200 gel purified the buPRL monomer. The purity of buPRL monomer was confirmed by 15% SDS PAGE. The buPRL monomer was >90% pure. It was characterized by specific anti-buPRL serum in ELISA and Western blot. A native PAGE of the PRL showed three charge isoforms. A protocol was standardized to separate prolactin monomeric least acidic isoforms using an anion exchanger.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Taylor and Francis Group. |
| Keywords: | Prolactin Monomer; Tryptophan; Sulphur |
| ID Code: | 64822 |
| Deposited On: | 17 Oct 2011 03:49 |
| Last Modified: | 17 Oct 2011 03:49 |
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