Prasad, Pramod V. ; Chaube, Shail K. ; Shrivastav, T. G. ; Kumari, G. L. ; Duraiswami, S. ; Muralidhar, K. (2005) Isolation of α- and β-subunits of peak-I hCG and generation of highly specific polyclonal antisera Journal of Immunoassay and Immunochemistry, 26 (4). pp. 345-355. ISSN 1532-4230
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Official URL: http://www.tandfonline.com/doi/abs/10.1080/1532181...
Related URL: http://dx.doi.org/10.1080/15321810500220985
Abstract
Development of polyclonal antisera is still a choice in some hard-pressed budget laboratories. In the present study, an attempt was made to isolate α- and β-subunits from peak-I hCG, generation of polyclonal antisera and their characterization. The anti-hCG-α antisera showed titres of 18000 and anti-hCG-β antisera 116,000 at 50% binding to radiolabelled hCG in RIA. Studies on specificity using anti hCG-β antiserum demonstrated no cross-reaction with several hormones tested in the present study, except for hCG-β and hCG, thus eliciting a highly specific hCG-β antiserum.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Taylor and Francis Group. |
| Keywords: | Peak-I hCG; Anti hCG-α; Anti hCG-β |
| ID Code: | 64819 |
| Deposited On: | 17 Oct 2011 03:48 |
| Last Modified: | 17 Oct 2011 03:48 |
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