Rajendra Kumar, T. ; Prakash, V. ; Muralidhar, K. (1990) Studies on buffalo pituitary lutropin (LH): physicochemical and immunological properties Journal of Protein Chemistry, 9 (6). pp. 727-733. ISSN 0277-8033
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Official URL: http://www.springerlink.com/content/k25h15h6176l71...
Related URL: http://dx.doi.org/10.1007/BF01024767
Abstract
Physicochemical and immunological properties of buffalo pituitary lutropin (buffalo LH) are reported here. The preparation was shown to be homogeneous by several physicochemical criteria. The molecular weight was found to be 30,000-40,000 by SDS-PAGE, GP-HPLC, and ultracentrifugation analyses. It showed certain interesting features, such as anomalous sedimentation behavior, microheterogeneity due to sugar-linked sulfate, and weak immunogenicity in rabbits. The subunit nature of the hormone has been confirmed. Sugar composition showed similarities as well as differences with LH of other species. Preliminary data on the homologous and heterologous RIAs, using iodinated sheep LH and buffalo LH and the respective antisera, have also been given.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Buffalo Pituitary Lutropin; Microheterogeneity; Sugar-linked Sulfate; Physicochemical Properties |
| ID Code: | 64802 |
| Deposited On: | 17 Oct 2011 03:46 |
| Last Modified: | 17 Oct 2011 03:46 |
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