Rajyalakshmi, M. ; Arunasmithasri, S. ; Srinivas, V. ; Muralidhar, K. (1983) Incorporation of radioactive 35SO2-4 into immunoreactive pituitary lutropin FEBS Letters, 154 (2). pp. 301-304. ISSN 0014-5793
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0014-5793(83)80171-9
Abstract
The terminal hexosamines of bovine pituitary lutropin are thought to contain a sulfate moiety. In order to test this, a biosynthetic approach was adopted. When rat and buffalo (bovine) pituitaries were incubated with radioactive 35SO2-4 for 2 h in vitro it was observed that radioactivity gets incorporated into trichloroacetic acid-precipitable proteins. When the radioactive proteins were treated with an anti-sheep lutropin serum, radoactivity was found in the immunoprecipitate. The incorporation into rat lutropin like material was very marginal while it was very significant in the case of buffalo lutropin.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Lutropin; Buffalo pituitary; Sulfate incorporation; LH antiserum; Rat LH; Immunoprecipitation |
| ID Code: | 64799 |
| Deposited On: | 15 Oct 2011 12:53 |
| Last Modified: | 15 Oct 2011 12:53 |
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