Sujatha, Sitaraman ; Chatterji, Dipankar (1999) Detection of putative Zn(II) binding sites within Escherichia coli RNA polymerase: inconsistency between sequence-based prediction and 65Zn blotting FEBS Letters, 454 (1-2). pp. 169-171. ISSN 0014-5793
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00145...
Related URL: http://dx.doi.org/10.1016/S0014-5793(99)00786-3
Abstract
The availability of repeating 'Cys' and/or 'His' units in a particular order prompts the prediction of Zn(II) finger motifs in a protein. Escherichia coli RNA polymerase has two tightly bound Zn(II) per molecule of the enzyme as detected by atomic absorption spectroscopy. One Zn(II) was identified to be at the β subunit, whereas the other putative Zn(II) binding site has recently been predicted to be at the N-terminal half of the β' subunit, from primary sequence analysis. We show here that the β' subunit has no ability to bind 65Zn(II). On the other hand, the N-terminal domain of the a subunit has strong Zn(II) binding ability with no obvious functional implications.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Biochemical Societies. |
| Keywords: | Zn(II) Site; Zn(II) Finger; 65Zn Blotting; Atomic Absorption; Subunit Location |
| ID Code: | 6434 |
| Deposited On: | 20 Oct 2010 10:24 |
| Last Modified: | 30 Sep 2011 10:10 |
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