Paenibacillus glacialis sp. nov., isolated from the Kafni glacier of the Himalayas, India

Abstract

A novel strain of the genus Paenibacillus, KFC91^T, was isolated from the Kafni glacier of the Himalayas. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KFC91^T clustered with Paenibacillus antarcticus LMG 22078^T (98.9%) and Paenibacillus macquariensis LMG 6935^T (98.7%). The cell wall peptidoglycan contained meso-diaminopimelic acid as the diamino acid, anteiso-C_15:0 as the predominant fatty acid and MK-7 as the major isoprenoid quinone. Based on the phylogenetic analysis, chemotaxonomic characteristics and other phenotypic traits, strain KFC91^T was assigned to the genus Paenibacillus. DNA-DNA hybridization experiments of KFC91^T with P. antarcticus and P. macquariensis showed reassociation values of 39 and 52%, respectively. Thus, it is proposed that strain KFC91^T should be assigned the status of a novel species of the genus Paenibacillus and the name proposed is Paenibacillus glacialis sp. nov., with KFC91^T (=NCCB 100252^T=DSM 22343^T) as the type strain.