Cesiribacter andamanensis gen. nov., sp. nov., isolated from a soil sample from a mud volcano

Abstract

A novel Gram-staining-negative, rod-shaped, non-motile bacterium, strain AMV16^T, was isolated from a soil sample collected from a mud volcano located in the Andaman Islands, India. The cell suspension was pale orange. Cells of strain AMV16^T were positive for catalase, oxidase, lipase, ornithine decarboxylase and lysine decarboxylase and negative for gelatinase and urease. The fatty acids present were anteiso-C_11:0 (5.4%), anteiso-C_12:0 (4.1%), C_12:0 (7.0%), iso-C_15:0 (14.4%), anteiso-C_15:0 (3.4%), anteiso-C_16:0 (3.0%), C_16:0 (2.6%), anteiso-C_17:0 (3.7%), iso-C_19:0 (9.7%), C_13:1 (13.8%), iso-C_15:1 G (15.9%), iso-C_16:1 G (11.1%) and summed feature 5 (anteiso-C_18:0 and/or C_18:2ω6,9c; 5.9%). Strain AMV16^T contained MK-4 as the major respiratory quinone and diphosphatidylglycerol and phosphatidylethanolamine made up the phospholipids. The G+C content of DNA of strain AMV16^T was 50.9 mol%. blast sequence similarity searches based on the 16S rRNA gene sequence indicated that species of the genus Marivirga were the nearest phylogenetic neighbours, with pairwise sequence similarity ranging from 89.9 to 90.0%. Phylogenetic analyses indicated that strain AMV16^T clustered with the type strains of Marivirga tractuosa and Marivirga sericea at a phylogenetic distance of 14.6% (85.4% similarity), distinct from clades representing other genera of the family 'Flammeovirgaceae'. Based on the above-mentioned phenotypic and phylogenetic characteristics, strain AMV16_T is proposed as a representative of a new genus and novel species, Cesiribacter andamanensis gen. nov., sp. nov. The type strain of Cesiribacter andamanensis is AMV16^T (=DSM 22818^T =CCUG 58431^T).