Agarwal, Sarita ; Khardori, Romesh ; Agarwal, S. S. (1990) Usefulness of MC-540 fluorescent dye as probe versus scanning electron microscopy for assessing membrane changes Toxicology Letters, 54 (2-3). pp. 169-174. ISSN 0378-4274
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0378-4274(90)90180-T
Abstract
The effect of primaquine enantiomers on cell membranes of glucose-6-phosphate (G-6PD)-deficient erythrocytes was studied in vitro. Staining with merocyanine (Mc-540) showed that exposure to primaquine enantiomers produces significant fluorescence in G-6PD-deficient erythrocytes, indicating marked drug-induced alterations in membrane fluidity. Scanning electron microscopy (SEM) studies confirmed that primaquine enantiomers altered membrane morphology (by producing stomatocytes) in both normal and G-6PD-deficient cells. The concentration-dependent effet, however, was more pronounced with MC-540, a lipophylic dye, than with SEM (an expensive technique).
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Erythrocyte membrane; MC-540; SEM; Primaquine |
| ID Code: | 64157 |
| Deposited On: | 05 Oct 2011 10:53 |
| Last Modified: | 05 Oct 2011 10:53 |
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