Ranjan, Amit ; Mallick, Birendra Nath (2010) A modified method for consistent and reliable Golgi-Cox staining in significantly reduced time Frontiers in Neurology, 1 . p. 157. ISSN 1664-2295
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Official URL: http://www.frontiersin.org/sleep_and_chronobiology...
Related URL: http://dx.doi.org/10.3389/fneur.2010.00157
Abstract
The two major limitations of Golgi-Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi-Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive, and provides uniform staining with very good resolution of neuronal soma, dendrites as well as spines.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Frontiers. |
| Keywords: | Golgi-Cox Stain; Incubation Temperature; Normal Body Temperature; Reduced Incubation Period; Thick Section; Thick Tissue Block |
| ID Code: | 63694 |
| Deposited On: | 29 Sep 2011 06:23 |
| Last Modified: | 29 Sep 2011 06:23 |
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