Rapid eye movement sleep deprivation decreases membrane fluidity in the rat brain

Abstract

In this study we examined the effects of rapid eye movement sleep (REMS) deprivation on synaptosomal and microsomal membrane fluidity by studying 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence polarization in control as well as REMS-deprived rats. The flower pot technique was used to perform 24, 48 and 96 h REMS deprivation. Suitable control experiments were carried out to rule out the nonspecific effects. The results showed that DPH fluorescence polarization increased both in the microsome as well as in the synaptosome in REMS-deprived animals, except in the cerebellum, indicating that there was a generalized decrease in membrane fluidity in the rat brain. The alterations in membrane fluidity returned to baseline upon recovery from REMS deprivation. Control experiments suggested that the alterations were primarily caused by REMS deprivation and not due to nonspecific effects. This finding supports REMS deprivation induced other changes reported earlier. This increase in membrane rigidity could be at least one of the possibilities for REMS loss induced alterations in physiological phenomena including membrane bound enzyme activities and receptor densities.