Gopal, Vijaya ; Kumar, K. Prasanna ; Chatterji, Dipankar (1988) Bovine seminal plasmin is a DNA unwinding protein FEBS Letters, 231 (2). pp. 389-392. ISSN 0014-5793
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...
Related URL: http://dx.doi.org/10.1016/0014-5793(88)80856-1
Abstract
The duplex DNA unwinding ability of seminal plasmin from bovine semen was examined by treatment of plasmid-protein complexes with calf thymus topoisomerase I and resolution of the topoisomer distributions by agarose gel electrophoresis. Binding of seminal plasmin results in a moderate degree of unwinding of supercoiled plasmid. The elongation of the RNA chain by E.coli RNA polymerase over promoter containing template is not inhibited by seminal plasmin. However, the reinitiation of transcription is blocked in such cases indicating that seminal plasmin inhibits transcription by binding to the initiation site of RNA polymerase.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Biochemical Societies. |
| Keywords: | Topoisomer; Unwinding; Seminal Plasmin; RNA Polymerase; Elongation |
| ID Code: | 6357 |
| Deposited On: | 20 Oct 2010 11:05 |
| Last Modified: | 30 Sep 2011 09:18 |
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