Chandra, Subhash ; Kaur, Manpreet ; Midha, Shuchi ; Gorantala, Jyotsna ; Bhatnagar, Rakesh (2007) Induction of cytotoxic T lymphocyte response against Mycobacterial antigen using domain I of anthrax edema factor as antigen delivery system Biochemical and Biophysical Research Communications, 357 (1). pp. 50-55. ISSN 0006-291X
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.bbrc.2007.03.166
Abstract
We have investigated the efficiency of N-terminal 1-260 residues of Edema factor (EFn) as a delivery system for ESAT-6, an antigenic protein of Mycobacterium tuberculosis H37Rv, into the cytosol of mammalian cells. The EFn.ESAT-6 recombinant protein was obtained by genetic fusion of EFn and ESAT-6 DNA. Our data shows that in the presence of PA, EFn.ESAT-6 fusion protein is internalized into the cytosol of antigen presenting cells, and the splenocytes produced both Th1 and Th2 cytokines in vitro. Further, EFn.ESAT-6 elicited effective cytotoxicT lymphocyte (CTL) response in an in vitro CTL assay. This study for the first time demonstrates that EFn can be used as a vehicle to deliver heterologous proteins of therapeutic importance.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Edema Factor; ESAT-6; Fusion Protein; CTL; Cytokines |
| ID Code: | 63346 |
| Deposited On: | 28 Sep 2011 10:29 |
| Last Modified: | 28 Sep 2011 10:29 |
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