Mukherjee, Raju ; Gomez, Manuel ; Jayaraman, Narayanaswamy ; Smith, Issar ; Chatterji, Dipankar (2005) Hyperglycosylation of glycopeptidolipid of mycobacterium smegmatis under nutrient starvation: structural studies Microbiology, 151 . pp. 2385-2392. ISSN 1350-0872
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Official URL: http://mic.sgmjournals.org/cgi/content/abstract/15...
Related URL: http://dx.doi.org/10.1099/mic.0.27908-0
Abstract
The presence of a polar species of glycopeptidolipid (GPL) in carbon-starved Mycobacterium smegmatis has been reported previously. In this study, the complete structure of this GPL is established with the help of MALDI-TOF (matrix assisted laser desorption/ionization time of flight) and ESI (electrospray ionization) -MS, 13C-SEFT (spin echo Fourier transform) -NMR spectroscopy, and HPLC analysis. In the molecule, two units of a 3,4-di-O-methyl derivative of rhamnose are attached to L-alaninol via a 1 → 2 linkage. Various methyl derivatives of rhamnose and 6-deoxytalose were synthesized as standards to establish this structure. The accumulation of this polar GPL in M. smegmatis is sigB dependent, as a SigB-overproducing strain of M. smegmatis shows the presence of this spot in the exponential phase, and a sigB-knockout strain of M. smegmatis does not show the presence of any polar GPLs.
Item Type: | Article |
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Source: | Copyright of this article belongs to Society for General Microbiology. |
ID Code: | 6301 |
Deposited On: | 20 Oct 2010 11:13 |
Last Modified: | 16 May 2016 16:38 |
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