Pahari, Susanta ; Chatterji, Dipankar (1997) Interaction of bacteriophage T4 AsiA protein with Escherichia coli σ70 and its variant FEBS Letters, 411 (1). pp. 60-62. ISSN 0014-5793
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00145...
Related URL: http://dx.doi.org/10.1016/S0014-5793(97)00668-6
Abstract
Bacteriophage T4 produces a small protein AsiA, which inhibits transcription from σ70-dependent promoters in E. coli by tightly binding to σ70 and is therefore termed as anti-sigma factor. We observed that there was no inhibition of single round transcription at lac UV5 promoter when AsiA was added to preformed open complex between RNA polymerase and template DNA. However, transcription was found to proceed normally at 'extended -10' promoters in the presence of AsiA. It appears therefore that AsiA binds σ70 at its 4.2-subdomain or in its close vicinity. Further experiments on immunoprecipitation of σ70 and a mutant σ70-V576G with AsiA seem to corroborate such conclusion.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Biochemical Societies. |
| Keywords: | AsiA; T4; σ70; Interaction; 4.2 Subdomain |
| ID Code: | 6295 |
| Deposited On: | 20 Oct 2010 11:15 |
| Last Modified: | 30 Sep 2011 10:07 |
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